This tutorial illustrates how to create a Minimum Spanning Tree (MST) based on MLST allele numbers. The same steps are also applicable for clustering of other categorical character data sets such as MLVA.
PDF file:
Download sample data:
Neisseria MLST data
MS Excel file, containing MLST allelic profiles for 500 Neisseria meningitidis strains, including serogroup and strain information.
Download demonstration database:
Neisseria MLST demo database
Demonstration database containing 500 Neisseria strains with sequences and allele IDs for the seven housekeeping genes, present the publicly available MLST scheme hosted at www.pubmlst.org.
Note that the downloaded database backup file (.bnbk) can be restored via the Restore database... functionality in the BIONUMERICS startup screen.