Denaturing Gradient Gel Electrophoresis of amplified 16S rDNA (commonly denoted as DGGE or PCR-DGGE) is a technique frequently used for bacterial community fingerprinting in microbial ecology. This tutorial illustrates how to import a DGGE gel image into your BIONUMERICS database, how to process the image, and how to calculate diversity indices on the imported DGGE profiles.
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DGGE sample data
Gel images containing DGGE profiles of bacterial 16S rDNA gene fragments that were amplified using proteobacteria specific primers.