wgMLST analysis

Whole-genome Multi-Locus Sequence Typing (wgMLST) using BIONUMERICS. See the wgMLST application page for a complete overview.

Hash-based whole genome MLST

This tutorial shows you how to work with the WGS tools local plugin, applied on the Listeria monocytogenes WGS demo database. The WGS tools local plugin performs a hash-based wgMLST allele calling and maintains a local wgMLST nomenclature. These features allow you to continue wgMLST analyses without a connection to the Calculation Engine.

fastq.gz files fail to upload to the CE Store

When trying to run a job on the Calculation Engine with fastq.gz files on a local file server, the fastq.gz files fail to upload to the CE Store and my job remains at status 'WaitingQueued' indefinitely.

3. Cluster analysis of wgMLST data

2. The Quality assessment window

1. Send calculation jobs to the external calculation engine

Installation of the WGS tools plugin and import of sequence read sets (as data links)

Whole genome MLST typing: routine workflow starting from sequence read sets

This tutorial explains how to prepare your database for wgMLST analysis and how to perform a full wgMLST analysis (de novo assembly, assembly-based and assembly-free calling) in BIONUMERICS on a routine basis starting from sequence read sets.

Whole genome MLST typing: routine workflow starting from imported genomes

This tutorial explains how to prepare your database for wgMLST analysis and how to perform a wgMLST analysis starting from genomes that are imported in BIONUMERICS.

Whole genome MLST typing: detailed exploration of results

In this tutorial the results from the de novo assembly and allele calling (assembly-free and assembly-based) are discussed into more detail.

Whole Genome Sequence tools plugin manual

In this manual you will learn how to install and use the WGS (Whole Genome Sequence) tools plugin in a BIONUMERICS database.

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wgMLST analysis