This tutorial shows you how to work with the WGS tools local plugin, applied on the Listeria monocytogenes WGS demo database. The WGS tools local plugin performs a hash-based wgMLST allele calling and maintains a local wgMLST nomenclature. These features allow you to continue wgMLST analyses without a connection to the Calculation Engine.
PDF file:
Download demonstration database:
WGS_demo_database_for_Listeria_monocytogenes
Demonstration database containing data for a set of 51 Listeria monocytogenes isolates. This database uses publicly available next-generation sequence reads from the Sequence Read Archive (SRA). For each isolate, NGS reads were de novo assembled into genome sequences. wgMLST alleles were called using the assembly-based and assembly-free method.
Note that the downloaded database backup file (.bnbk) can be restored via the Restore database... functionality in the BIONUMERICS startup screen.